Journal: Microbial Pathogenesis

Article Title: Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

doi: 10.1016/j.micpath.2020.104560

Figure Lengend Snippet: Representative results showing distributions of (A) CD3 + CD4 + and (B) CD3 + CD8 + T-lymphocytes from IB DNA vaccinated chickens (group E) as determined by flow cytometry.

Article Snippet: A single cell suspension was prepared at a concentration of 1 × 10 7 cells/ml and about 100 μl of cell suspensions (1 × 10 6 cells) were incubated for 2 h at 4 °C with mouse anti-chicken CD3-FITC (Southern Biotech, USA), mouse anti-chicken CD4-R-phycoerythrin (R-PE) (Southern Biotech, USA), and mouse anti-chicken CD8 cy5 (Southern Biotech, USA).

Techniques: Flow Cytometry

Journal: Microbial Pathogenesis

Article Title: Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

doi: 10.1016/j.micpath.2020.104560

Figure Lengend Snippet: Percentage CD3 + CD4 + and CD3 + CD8 + T-lymphocytes in vaccinated and control chicken groups 2 weeks after booster vaccination with IB - DNA vaccines. Vaccination regimen and control includes: (A) PBS; (B) pBudCE4.1 (naked DNA); (C) pBudCR88-S1; (D) pBudM41-S1; (E) pBudCR88S1/M41S1+Nano; (F) pBudCR88S1/M41S1- Nano. Evaluations of Clinical signs and viral shedding after challenge.

Article Snippet: A single cell suspension was prepared at a concentration of 1 × 10 7 cells/ml and about 100 μl of cell suspensions (1 × 10 6 cells) were incubated for 2 h at 4 °C with mouse anti-chicken CD3-FITC (Southern Biotech, USA), mouse anti-chicken CD4-R-phycoerythrin (R-PE) (Southern Biotech, USA), and mouse anti-chicken CD8 cy5 (Southern Biotech, USA).

Techniques: Control, Vaccines